| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcGHLT A |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8757 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcGHLTA载体基本信息
pAcGHLTA载体图谱质粒图谱和多克隆位点信息
pAcGHLTA载体简介
pAcGHLTA载体序列
LOCUS pAcGHLT A 8757 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal GST-6xHis-PKA-thrombin cassette. For other reading
frames, use pAcGHLT B or pAcGHLT C.
ACCESSION .
VERSION .
KEYWORDS pAcGHLT A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8757)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8757
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2206..2859
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 2875..2892
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2899..2916
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2926..2943
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2958..3029
/note="MCS"
/note="multiple cloning site"
misc_recomb 3049..4507
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(3166..4507)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5880..6335
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6617..6721
/gene="bla"
/note="AmpR promoter"
CDS 6722..7582
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7753..8341
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
2941 agcccaggac tcgatggcat atgctcgatc gaggaattca ggcctccatg ggagctcgcg
3001 gccgcctgca gggtaccccc gggagatctg taccgactct gctgaagagg aggaaattct
3061 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
3121 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
3181 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
3241 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
3301 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
3361 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
3421 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
3481 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
3541 agtgtacaat tgactcgacg taaacacgtt aaataaagct tggacatatt taacatcggg
3601 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
3661 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
3721 cgcgatcaaa tttgtagttg agctttttgg aattatttct gattgcgggc gtttttgggc
3781 gggtttcaat ctaactgtgc ccgattttaa ttcagacaac acgttagaaa gcgatggtgc
3841 aggcggtggt aacatttcag acggcaaatc tactaatggc ggcggtggtg gagctgatga
3901 taaatctacc atcggtggag gcgcaggcgg ggctggcggc ggaggcggag gcggaggtgg
3961 tggcggtgat gcagacggcg gtttaggctc aaatgtctct ttaggcaaca cagtcggcac
4021 ctcaactatt gtactggttt cgggcgccgt ttttggtttg accggtctga gacgagtgcg
4081 atttttttcg tttctaatag cttccaacaa ttgttgtctg tcgtctaaag gtgcagcggg
4141 ttgaggttcc gtcggcattg gtggagcggg cggcaattca gacatcgatg gtggtggtgg
4201 tggtggaggc gctggaatgt taggcacggg agaaggtggt ggcggcggtg ccgccggtat
4261 aatttgttct ggtttagttt gttcgcgcac gattgtgggc accggcgcag gcgccgctgg
4321 ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg ggtggtggca attcaatatt
4381 ataattggaa tacaaatcgt aaaaatctgc tataagcatt gtaatttcgc tatcgtttac
4441 cgtgccgata tttaacaacc gctcaatgta agcaattgta ttgtaaagag attgtctcaa
4501 gctcggatcg atcccgcacg ccgataacaa gccttttcat ttttactaca gcattgtagt
4561 ggcgagacac ttcgctgtcg tcgacgtaca tgtatgcttt gttgtcaaaa acgtcgttgg
4621 caagctttaa aatatttaaa agaacatctc tgttcagcac cactgtgttg tcgtaaatgt
4681 tgtttttgat aatttgcgct tccgcagtat cgacacgttc aaaaaattga tgcgcatcaa
4741 ttttgttgtt cctattattg aataaataag attgtacaga ttcatatcta cgattcgtca
4801 tggccaccac aaatgctacg ctgcaaacgc tggtacaatt ttacgaaaac tgcaaaaacg
4861 tcaaaactcg gtataaaata atcaacgggc gctttggcaa aatatctatt ttatcgcaca
4921 agcccactag caaattgtat ttgcagaaaa caatttcggc gcacaatttt aacgctgacg
4981 aaataaaagt tcaccagtta atgagcgacc acccaaattt tataaaaatc tattttaatc
5041 acggttccat caacaaccaa gtgatcgtga tggactacat tgactgtccc gatttatttg
5101 aaacactaca aattaaaggc gagctttcgt accaacttgt tagcaatatt attagacagc
5161 tgtgtgaagc gctcaacgat ttgcacaagc acaatttcat acacaacgac ataaaactcg
5221 aaaatgtctt atatttcgaa gcacttgatc gcgtgtatgt ttgcgattac ggattgtgca
5281 aacacgaaaa ctcacttagc gtgcacgacg gcacgttgga gtattttagt ccggaaaaaa
5341 ttcgacacac aactatgcac gtttcgtttg actggtacgc ggcgtgttaa catacaagtt
5401 gctaaccggc ggccgacacc catttgaaaa aagcgaagac gaaatgttgg acttgaatag
5461 catgaagcgt cgtcagcaat acaatgacat tggcgtttta aaacacgttc gtaacgttaa
5521 cgctcgtgac tttgtgtact gcctaacaag atacaacata gattgtagac tcacaaatta
5581 caaacaaatt ataaaacatg agtttttgtc gtaaaaatgc cacttgtttt acgagtagaa
5641 ttagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
5701 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
5761 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
5821 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
5881 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
5941 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
6001 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
6061 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
6121 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
6181 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
6241 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
6301 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
6361 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
6421 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
6481 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
6541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
6601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
6661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
6721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
6781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
6841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
6901 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
6961 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
7021 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
7081 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
7141 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
7201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
7261 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
7321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
7381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
7441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
7501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
7561 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
7621 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
7681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
7741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
7801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
7861 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
7921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
7981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
8041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
8101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
8161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
8221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
8281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
8341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
8401 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
8461 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
8521 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
8581 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
8641 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
8701 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaatt
//
